Phase 3 ENG

Phase no. 3/2016:

Completion of ex situ collections – partial. Elaboration of the functional models for the in vitro preservation technologies – partial

Period of 12 months: 01.01.2016 – 31.12.2016


The objective of TEHNAT project is the adaptation and/or optimization of some biotechnologies of ex situ multiplication and conservation and of molecular techniques – SSR and ISSR – for 3 endemic/endangered Caryophyllaceae species: Lychnis nivalis Kit., endemic species for Rodnei Mts., considered rare and vulnerable, Silene dinarica Spreng, endemic for the South of Carpathian Mts., rare species and Moehringia jankae Griseb. ex Janka from the North of Dobrogea, considered rare and vulnerable. The species chorology and the in situ conservation condition of the species populations was done, as well as the elaboration, following project’s results, of several recommendations for the consolidation of Natura 2000 network.

The objectives of Phase 3 were entirely accomplished. All the 4 partner institutions took part in achieving all the activities of this phase, having a good cooperation between the state institutions and the company. 21 researchers took part in phase 3, among which 5 young postdoctoral researchers. The following activities were achieved during this phase:

3.1. Achieving the functional model of in vitro culture technology for the studied taxa – partial. The functional model of in vitro culture technology was achieved based on the experimental model elaborated in phases 2 and 3. The functional model contains the technologies of: aseptic cultures initiation, their multiplication and stabilization, and rhizogenesis.

3.2. Achieving the functional model for the in vitro conservation technology on medium term. This was elaborated for all the 3 taxa. According to our experiments, different factors for the control of growth, development and viability, on medium term, can be applied, depending on taxon.

3.3. Achieving the acclimatization methodology. The vitroplants were adapted to ex vitro culture conditions, having pot plants cultivated in soil and then, cultivated in open-air.

3.4. Assessment of in situ conservation condition – partial. In addition to the former studies, 30 new locations were studied for S. dinarica and 5 new sites (10 relevées) for M. jankae.

3.5. Achieving the functional models of SSR and ISSR molecular techniques for the study of population genetic variability – partial. SSR markers for S. dinarica show an extremely reduced genetic polymorphism and for M. jankae is missing. ISSR markers show a high level of genetic polymorphism in both species.

3.6. Elaboration of in vitro multiplication experimental model – partial. The experimental model for rhizogenesis of L. nivalis and S. dinarica was elaborated within this phase of the project.


The results of the phase consisted of: 4 new technologies, 1 product, 1 information product, 4 attendances to conferences, among which 1 international, all with published summaries (with Acknowledgement), 1 BDI published paper and 3 papers in evaluation for ISI journals.