Phase 2 ENG

PHASE NO 2/2015:

The evaluation of in situ conservation status of taxa. The study of genetic polymorphism. Ex situ conservation of the endemic/endangered studied taxa – phase partially accomplished in 2015 and to be continued in 2016
Period of 12 months: 01.01.2015 – 31.12.2015

PHASE 2/2015 SUMMARY

The Phase 2 objectives were totally accomplished. All the 4 institutions, including the company took part to the objectives activities, having good cooperation. The achievement of this phase implied 26 researchers among which 6 young postdoctoral researchers. The following activities were done within this phase of the project:
Activity 2.1. IN SITU IDENTIFICATION OF POPULATIONS FOR THE ENDEMIC/ENDANGERED STUDIED CARYOPHYLLACEAE TAXA

This activity implied the field research begun in 2014. In 2015 there were in situ identified all the studied species:

– Lychnis nivalis, endemic species in Rodnei Mts., considered rare and vulnerable – was identified in 19 locations
Lychnis nivalis Kit. in Lala Valley, under Inău Mt., 29.07.2015 (photo Stoica Ilie-Adrian)

– Silene dinarica, endemic in the South of Carpathian Mts., considered rare – was identified in 10 locations

S. dinarica in the area of Scarisoara Lake – Fagaras Mts. (photo: O. Gavrilas – 21.07.2015)

– Moehringia jankae, form the North of Dobrogea, considered rare and vulnerable – was identified in 8 locations.
Moehringia jankae, Dobrogea – in Atmagea area (photo: I. Holobiuc, P. Anastasiu – 2015)
During these field trips, there was sampled plant material for the initiation of ex situ collections, for the study of the populations’ genetic variability and seeds.
Activity 2.2. THE FUNCTIONAL MODELS OF THE SSR AND ISSR TECHNIQUES OF MOLECULAR ANALYSIS FOR STUDYING THE POPULATIONS GENETIC VARIABILITY – partially done in 2015 and to be continued in 2016

The SSR (simple sequence repeats) and ISSR (inter simple sequence repeats) markers based on DNA analysis were used within this activity. Among SSR and ISSR markers were used some of the previously developed markers for Dianthus genus, but also markers developed for different Silene species. There were studied 5 populations of Silene nivalis, with 10-20 individuals each. As outgroup species was studied Silene acaulis.
The genetic polymorphism relieved by means of SSR markers is extremely low, especially for the S. nivalis populations from Lala, Galaţi and Stiol. The ISSR markers revealed a higher level of polymorphism in case of populations form Lala and Stiol, and in case of populations from Inău and Galaţi.
Activity2.3. ELABORATION OF EXPERIMENTAL MODEL FOR THE IN VITRO MULTIPLICATIONS OF THE STUDIED TAXA – partially done in 2015 and to be continued in 2016

There were settled the phases of the experimental model by in vitro culture technology to all the 3 studied species, as well as the experimental device. 12 experimental culture medium variants were studied in case of L. nivalis, the maximum multiplication rate being 30 neoplants/inoculum; in case of S. dinarica – 9 culture medium variants, multiplication rate of 16 neoplants/inoculum, and in case of M. jankae – 8 culture medium variants, multiplication rate of 14 neoplants/inoculum.

L. nivalis and S. dinarica after 9 weeks from the transfer on different multiplication media tested for the elaboration of the in vitro multiplication experimental model – BAP 1mg/l and ANA 0,2 mg/l culture media

M. jankae after 8 weeks from the transfer on different multiplication media tested for the elaboration of the in vitro multiplication experimental model – TDZ and IBA culture media
THE RESULTS obtained within these activities are:
– chorological digital maps for each species,
– the functional model of SSR and ISSR techniques for analyzing the populations genetic variability of L. nivalis, and
– experimental models of in vitro multiplication for the 3 studied species.

Within the framework of this phase, the researchers attended 6 conferences, among which 3 international. There were also written 3 scientific papers which will be published in ISI journals.